Recombinant Mouse FOLR4/Juno Protein

來源: 發布時間:2024-05-29

分子特性:牛凝血酶的分子特性符合高比活度酶的標準,純度達到SDS-PAGE電泳無雜蛋白條帶的要求。穩定性:牛凝血酶在室溫下運輸穩定,在2~8℃條件下可保存3年而酶活力未有變化。應用效果:在1:2000的質量比下,牛凝血酶能有效切割蛋白,適用于科研和工業生產中的重組融合蛋白特異性斷裂。討論科研應用:牛凝血酶因其高比活度和專一性,在分子生物學、生物化學和生物工程制藥研究中作為工具酶具有重要價值。臨床:作為止血藥物,牛凝血酶在外科手術中的應用超過100年,因其簡便和高效而受到青睞6。生產優勢:牛凝血酶的生產穩定性好,純度高,不含有其他蛋白酶活性,適合大規模生產和應用。全長跨膜蛋白A2AR,也就是腺苷受體A2aR(ADORA2A),是一種七次跨膜蛋白,屬于G蛋白偶聯受體。Recombinant Mouse FOLR4/Juno Protein,His Tag

Recombinant Mouse FOLR4/Juno Protein,His Tag,標準物質

RecombinantBiotinylatedHumanHLA-A*03:01&B2M&KRASG12V(VVGAVGVGK)MonomerProtein,His-AviTag性能參數分子別名(Synonyms)MHC;KRAS;K-Ras2;KRAS2;C-K-RAS;CFC2;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;KRAS1;KRAS2;NS;NS3;RASK2;GTPaseKras;KI-RAS;RALD表達區間及表達系統(Source)BiotinylatedHumanHLA-A*03:01&B2M&KRASG12V(VVGAVGVGK)MonomerProteinisexpressedfromHEK293withHistagandAvitagattheC-TerminusItcontainsGly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M)andVVGAVGVGKpeptide.[Accession|NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVGAVGVGK]分子量大小(MolecularWeight)TheproteinhasapredictedMWof50.09kDa.Duetoglycosylation,theproteinmigratesto51-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Suppliedas0.22μmfilteredsolutioninPBS(pH7.4).Recombinant Human SHH全長A2aR蛋白具有天然完整構象,VLP(病毒樣顆粒)固有特征可以帶來更高的免疫原性。

Recombinant Mouse FOLR4/Juno Protein,His Tag,標準物質

SARS-CoV-2,whichcausestheglobalpandemiccoronavirusdisease2019(Covid-19),belongstoafamilyofvirusesknownascoronaviruses.TheSARS-CoV-2Sproteinisaglycoproteinthatmediatesmembranefusionandviralentry.TheRBDofSARS-CoV-2bindsametallopeptidase,angiotensin-convertingenzyme2(ACE-2).SeveralemergingSARS-CoV-2genomeshavebeenidentifiedincludingtheOmicron,orB.1.1.529,variant.FirstidentifiedinNovember2021inSouthAfrica,theOmicronvariantquicklybecamethepredominantSARS-CoV-2variantandisconsideredavariantofconcern(VOC).TheOmicronvariantcontains15mutationsinRBDdomainthatpotentiallyaffectviralfitnessandtransmissibility.ThemajorityofthemutationsareinvolvedinACE-2bindingandOmicronbindsACE-2withgreateraffinity,potentiallyexplainingitsincreasedtransmissibility.Severalofthesemutationsarealsoidentifiedinfacilitatingimmuneescapeandreducingneutralizationactivitytoseveralmonoclonalantibodies.

RecombinantBiotinylatedHumanOX40/TNFRSF4/CD134Protein(PrimaryAmineLabeling),hFcTag性能參數分子別名(Synonyms)OX40Lreceptor;CD134;TNFRSF4;Ly-70表達區間及表達系統(Source)BiotinylatedHumanOX40/TNFRSF4/CD134Protein(PrimaryAmineLabeling)isexpressedfromHEK293withhFctagattheC-Terminus.ItcontainsLeu29-Ala216.[Accession|P43489]分子量大小(MolecularWeight)TheproteinhasapredictedMWof46.8kDa.Duetoglycosylation,theproteinmigratesto72-75kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGE制劑(Formulation)Suppliedas0.22μmfilteredsolutioninPBS(pH7.4).儲存條件Theproductshouldbestoredat-85~-65℃for1yearfromdateofreceipt.Recommendtoaliquottheproteinintosmallerquantitieswhenfirstusedandavoidrepeatedfreeze-thawcycles.PNGase F是一種酰胺水解酶,能夠特異性地裂解糖蛋白中由天冬酰胺連接的高甘露糖、雜合和復雜型的寡糖。

Recombinant Mouse FOLR4/Juno Protein,His Tag,標準物質

RecombinantBiotinylatedHumanKIR2DL1Protein,His-AviTag性能參數分子別名(Synonyms)CD158A;CD158Ankat1;cl-42表達區間及表達系統(Source)BiotinylatedHumanKIR2DL1ProteinisexpressedfromHEK293withHistagandAvitagattheC-Terminus.ItcontainsHis22-Arg242.[Accession|P43626]分子量大小(MolecularWeight)TheproteinhasapredictedMWof27.1kDa.Duetoglycosylation,theproteinmigratesto48-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.儲存條件Theproductshouldbestoredat-25~-15℃for1yearfromdateofreceipt.2-7days,2~8°Cundersterileconditionsafterreconstitution.2aR蛋白在細胞內發揮著不同的作用,例如在信號傳遞、物質轉運和細胞通訊等方面。Recombinant Human HSP70 Protein,His Tag

全長A2aR蛋白可應用于免疫、ELISA、SPR(表面等離子共振)、BLI(生物層干涉)和細胞實驗等場景。Recombinant Mouse FOLR4/Juno Protein,His Tag

重組型TEV蛋白酶(rTEV)是經過基因工程改造和純化后的重組蛋白酶,不僅保持天然TEV酶的功能活性,且在廣譜的溫度范圍內表現出更強的穩定性和特異性。rTEV是一種用來切除融合蛋白上親和標簽的常用工具酶,具有很強的位點特異性,嚴格識別七氨基酸序列EXXYXQ↓(G/S),切割位點在谷氨酰胺和甘氨酸/絲氨酸之間。普遍應用的七氨基酸序列為:Glu-Asn-Leu-Tyr-Phe-Gln↓-Gly。rTEV在pH7.0,30℃時可達活性,但在pH6.0-8.5和溫度4-30℃范圍內皆有活性(見表1),使得反應條件的選擇可根據目的蛋白的情況而靈活變動。另外rTEV切割后也能利用其N端的6×His標簽,通過Ni-NTA樹脂去除,以達到純化目的蛋白的目的。產品性質來源(Source)大腸桿菌外觀(Appearance)無色透明液體比活力(SpecificActivity)5U/μL活性定義(UnitDefinition)在1×rTEVBuffer(50mMTris,pH8.0,0.1mMEDTA,1mMDTT)中,30℃反應1h,剪切>85%的3μg底物所需要的酶量定義為一個活性單位。純化(Purification)Ni柱親和純化純度(Purity)≥95%(bySDS-PAGE)產品組分運輸與保存方法冰袋運輸。Recombinant Mouse FOLR4/Juno Protein,His Tag

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